Studies on Nucleoside Deaminase
نویسندگان
چکیده
منابع مشابه
Studies on porphobilinogen-deaminase from Saccharomyces cerevisiae.
Porphobilinogen-deaminase from Saccharomyces cerevisiae has been isolated and partially purified 80- and 230-fold in the absence or presence of phenylmethylsulphonyl fluoride, respectively. Some properties of the isolated enzyme were studied. Porphyrin formation was linear with time and protein concentration. Optimum pH was about 7.5-7.8. Molecular mass of the protein was 30,000 +/- 3000 Dalton...
متن کاملCombined familial adenosine deaminase and purine nucleoside phosphorylase deficiencies.
We studied an Arab family in which two infants died of severe combined immunodeficiency caused by adenosine deaminase (ADA) deficiency. One infant had purine nucleoside phosphorylase (PNP) activity in the leucocytes only half that of normal. Four other infant siblings had previously died from infections before the age of 2 months. Hyperpigmented skin lesions preceded death in three cases. The h...
متن کاملPurine Nucleoside Phosphorylase ( PNP ) and Adenosine Deaminase ( ADA ) Activities
New techniques have been devised for the cytochemical demonstration of purine nucleoside phosphorylase (PNP) and adenosine deaminase (ADA) activities in unfixed human lymphocytes. A suspension of living lymphocytes is mixed with agarose sol containing the reagents for the detection of PNP or ADA activity on a glass slide. The mixture solidifies. is incubated, and then dried for lightmicroscopic...
متن کاملLymphospecific toxicity in adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency: possible role of nucleoside kinase(s).
Inherited deficiencies of the enzymes adenosine deaminase (adenosine aminohydrolase; EC 3.5.4.4) and purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase; EC 2.4.2.1) preferentially interfere with lymphocyte development while sparing most other organ systems. Previous experiments have shown that through the action of specific kinases, nucleosides can be "trapped"...
متن کاملBiochemical studies of the nucleoside analogue, formycin.
The absorption and fluorescence properties of formycin provide a useful tool in the analysis of enzyme-substrate interactions; we have therefore studied selected biochemical properties of formycin nucleotides. Formycin and its derivatives effectively substitute for the corresponding adenosine substrates in a wide variety of enzyme reactions. These include enzymes of nucleotide metabolism, RNA p...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43487-x